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KMID : 0613820200300100912
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Journal of Life Science
2020 Volume.30 No. 10 p.912 ~ p.918
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Development of a Microsatellite Marker Set for the Individual Identification and Parentage Verification of Korean Native Black Goats
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Lee Sang-Hoon
Kang Ho-Chan
Lee Sung-Soo
Lee Jin-Wook
Kim Eun-Ho
Myung Hyun-Cheol
Kim Kwan-Woo
Lim Hyun-Tae
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Abstract
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The Korean native black goat (Capra hircus coreanae) is the goat species to be officially registered in Korea under the Food and Agriculture Organization. The object of this study is to establish a set of microsatellite (MS) markers for the individual identification and parentage verification of goats. In this study, we analyzed alleles of MS markers in crosses between Korean native black goats and crossbred goats (n=304 animals), and, based on the diversity of alleles for each marker, we selected 11 MS markers for individual identification and parentage verification. Using these 11 MS markers, the probabilities of different individuals with the same genotype being found within random and half-sib mating populations were 5.58×10-10 and 1.15×10-7, respectively. The parentage verification accuracy was 0.999996 when information about the parents was available and 0.999833 with no information. Thus, even given the total rearing population of 576,150 animals in South Korea, we concluded that these markers could be used for the individual identification and parentage verification of goats. Moreover, by analyzing the genetic relationships between the four lines of Korean native black goats and the crossbred goats, we verified the genetic characteristics of Korean native black goats, confirming their conservation value as a unique genetic resource.
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KEYWORD
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Goat, identification, microsatellite marker, multiplex PCR, parentage test
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